Cellometer K2

Artikelnr.:
CETK2
Preis: Sie sind nicht eingeloggt

The Cellometer K2 is specifically optimized for analysis of primary cells from peripheral blood, cord blood, bone marrow, and other complex samples for use in a wide range of research areas, including:

  • Nucleated Cells for Transplantation
  • PBMCs for Immunology
  • Splenocytes for Vaccine Development
  • Stem Cells for Cellular Therapy
  • Tumor Cell Suspensions for Oncology

Dual-color fluorescence allows for staining of live and dead nucleated cells, generating accurate viability results even in the presence of debris, platelets, and red blood cells. Accurate analysis of both 'messy' and 'clean' samples enables the K2 to evaluate samples at a variety of points throughout sample processing - from initial collection, to separation, to cryopreservation.

In addition, the Cellometer K2 can be certified for use in GxP regulated environments.

  • Features & Applications
  • Specifications

Primary Cell Analysis: PBMCs, Hepatocytes, and more

The Cellometer K2 is specifically optimized for analysis of primary cells from peripheral blood, cord blood, bone marrow, and other complex samples for use in a wide range of research areas, including:

  • Nucleated Cells for Transplantation
  • PBMCs for Immunology
  • Splenocytes for Vaccine Development
  • Stem Cells for Cellular Therapy
  • Tumor Cell Suspensions for Oncology

Dual-color fluorescence allows for staining of live and dead nucleated cells, generating accurate viability results even in the presence of debris, platelets, and red blood cells. Accurate analysis of both 'messy' and 'clean' samples enables the K2 to evaluate samples at a variety of points throughout sample processing - from initial collection, to separation, to cryopreservation.

The Cellometer K2 features assays for analysis of a wide range of primary samples, including:

hepatocyte cells Jurkat cells

              Hepatocytes                                              Jurkat

Splenocytes PBMC

              Splenocytes                                                 PBMC

Live / Dead Nucleated Cell Counts using Dual-Fluorescence

Green fluorescent live cell image

Green fluorescent live cell image

Red fluorescent dead cell image

Red fluorescent dead cell image

Why Dual-Fluorescence?

Because bright field cell counting does not differentiate nucleated from non-nucleated cells and trypan blue staining is not as easy to detect as fluorescent staining, dual-color fluorescence is strongly recommended for accurate viability analysis for primary cells. The K2 is equipped with standard assays for dual-fluorescence analysis of primary cells stained with AO/PI.

The AO/PI Method

Acridine Orange, AO, is a nuclear staining (nucleic acid binding) dye permeable to both live and dead cells. It stains all nucleated cells to generate green fluorescence. Propidium iodide, PI, can only enter dead cells with compromised membranes. It stains all dead nucleated cells to generate red fluorescence. Cells stained with both AO and PI fluoresce red due to quenching, so all live nucleated cells fluoresce green and all dead nucleated cells fluoresce red.

 

Lieferumfang:

  • Cellometer K2 Instrument
  • zwei Standard Fluoreszenzoptik-Module
  • Cellometer Software
  • Netzteil
  • USB 2.0 Verbindungskabel*
  • Telefon- und Onlinesupport bei der Installation

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Optionen:

  • PC/Laptop  passend erhältlich auf Anfrage

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Imaging Performance:

Zellgröße: 5-300* µm 
Konzentrationsbereich: 10^5-10^7 Zellen/ml

Hellfeld-Abbildung mit Bilderkennungssoftware für die schnelle und akkurate Zählung individueller Zellen. 

*Cellometer CHT4-PD300 Slides benötigt für Zellen von über 80 µm

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Gerätespezifikationen:

Gewicht: 10,4 kg

Breite: 15,6 cm
Tiefe: 21,6 cm
Höhe: 35,6 cm

Leistungsaufnahme Netzteil: 100-240 V Wechselstrom, 50/60 Hz, 1 A
Geräteversorgung: 12 V Gleichstrom, 3,34 A

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Standard Fluoreszenzoptik-Module

VC-535-402
Excitation / Emission: 470nm/535nm
Fluorophor-Beispiele:

  • Acridine Orange
  • CFDA
  • FITC

VC-660-502
Excitation / Emission: 540nm/660nm 
Fluorophor-Beispiele:

  • Propidium Iodide (PI)
  • Ethidium Bromide
  • 7-AAD

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*Minimal empfohlene Computer-Spezifikationen:
2.2 GHz oder mehr
4GB RAM
USB 2.0 port
1024 x 768 pixel display 
Windows XP, Vista, Windows 7

 

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